What would happen if you did not add acid after exactly 15 minutes of the enzymatic reaction in a competition ELISA?

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  • 2 months ago

    Acid is used to stop the reaction taking place because it inactivates the enzyme. If you don't add the acid at the correct time, the reaction will continue and more product will be made.If you then compare this result to others where the acid was added at the correct time, this result will be different because of the different time of reactin.

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