How to dilute a compound with a certain DMSO concentration?
For an assay, I need to take a 10mM stock of a compound (in 100% DMSO) and get it down to 1um in 0.1% DMSO in the well of a 96-well plate. 80 ul of substrate and 10 ul of NADPH also go into the well, so I guess I'll need 10 ul of 10 um in 1% DMSO to put into the well? How would I go about making the appropriate dilutions to do this? Thanks.
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